Development of sensitive techniques for rapid detection of viruses is on a high demand. Surface‐enhanced Raman spectroscopy (SERS) is an appropriate tool for new techniques due to its high sensitivity. DNA aptamers are short structured oligonucleotides that can provide specificity for SERS biosensors. Existing SERS‐based aptasensors for rapid virus detection had several disad-vantages. Some of them lacked possibility of quantitative determination, while others had sophisti-cated and expensive implementation. In this paper, we provide a new approach that combines rapid specific detection and the possibility of quantitative determination of viruses using the example of influenza A virus. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.