COVID-19.рф: ИНФОРМАЦИЯ ПРОТИВ ПАНДЕМИИ

Поиск по репозиторию

Статья

Development and Evaluation of a One-Step Quantitative RT-PCR Assay for Detection of Lassa Virus

V. Dedkov, N. Magassouba, M. Safonova, E. Naydenova, A. Ayginin, B. Soropogui, F. Kourouma, A. Camara, J. Camara, A. Kritzkiy, I. Tuchkov, M. Shchelkanov, V. Maleev,
2021
https://doi.org/10.1016/j.jviromet.2019.113674

Lassa fever is a severe viral hemorrhagic illness caused by Lassa virus. Based on estimates, the number of LASV infections ranges from 300,000 to 500,000 cases in endemic areas with a fatality rate of 1%. Development of fast and sensitive tools for the control and prevention of Lassa virus infection as well as for clinical diagnostics of Lassa fever are crucial. Here we reported development and evaluation of a one-step quantitative RT-qPCR assay for the Lassa virus detection – LASV-Fl. This assay is suitable for the detection of lineages I-IV of Lassa virus. The limit of detection of the assay ranged from 103 copies/ml to 105 copies/ml and has 96.4% diagnostic sensitivity, whereas analytical and diagnostic specificities both were 100%. Serum, whole blood and tissue are suitable for use with the assay. The assay contains all the necessary components to perform the analysis, including an armored positive control (ARC+) and an armored internal control (IC). The study was done during the mission of specialized anti-epidemic team of the Russian Federation (SAET) in the Republic of Guinea in 2015-2018. Based on sequencing data, LASV-specific assay was developed using synthetic MS2-phage-based armored RNA particles, RNA from Lassa virus strain Josiah, and further, evaluated in field conditions using samples from patients and Mastomys natalensis rodents. © 2019 Elsevier B.V.

Цитирование

Похожие публикации

One-step quantitative RT-PCR assay with armored RNA controls for detection of SARS-CoV-2
E. Goncharova, V. Dedkov, A. Dolgova,
Development of one-step real-time PCR assay for titrating trivalent live attenuated influenza vaccines
Y. Zang, D. Du, P. Ge,
Development of the kit for diagnostics of COVID-19 by real time RT-PCR
A. Shuryaeva, T. Malova, E. Davydova,

Документы

Источник

Версии

  • 1. Version of Record от 2021-04-27

Метаданные

Об авторах
  • V. Dedkov
    Pasteur Institute, Federal Service on Consumers’ Rights Protection and Human Well-Being Surveillance, Saint-Petersburg, Russian Federation
  • N. Magassouba
    Martsinovsky Institute of Medical Parasitology, Tropical and Vector Borne Diseases, Sechenov First Moscow State Medical University, Moscow, Russian Federation
  • M. Safonova
    Laboratorie de Virologic B1568, Université Gamal Abdel Nasser de Conakry, Projet de Recherche sur les Fièvres Hémorragiques en Guinée, Conakry, Guinea
  • E. Naydenova
    Anti-Plague Center, Federal Service on Consumers’ Rights Protection and Human Well-Being Surveillance, Moscow, Russian Federation
  • A. Ayginin
    Russian Research Anti-Plague Institute «Microbe», Federal Service on Consumers’ Rights Protection and Human Well-Being Surveillance, Saratov, Russian Federation
  • B. Soropogui
    Central Research Institute for Epidemiology, Federal Service on Consumers’ Rights Protection and Human Well-Being Surveillance, Moscow, Russian Federation
  • F. Kourouma
    Far Eastern Federal University, Vladivostok, Russian Federation
  • A. Camara
  • J. Camara
  • A. Kritzkiy
  • I. Tuchkov
  • M. Shchelkanov
  • V. Maleev
Название журнала
  • Journal of Virological Methods
Том
  • 271
Страницы
  • -
Ключевые слова
  • adolescent; adult; Article; child; controlled study; cross reaction; female; human; Lassa virus; limit of detection; male; Mastomys natalensis; middle aged; nonhuman; priority journal; quantitative analysis; real time polymerase chain reaction; school child; sensitivity and specificity; virus detection; virus strain; young adult; animal; blood; DNA probe; genetics; Guinea; isolation and purification; Lassa fever; Lassa virus; murine; procedures; real time polymerase chain reaction; virology; primer DNA; virus RNA; Adolescent; Adult; Animals; Child; DNA Primers; DNA Probes; Female; Guinea; Humans; Lassa Fever; Lassa virus; Limit of Detection; Male; Middle Aged; Murinae; Real-Time Polymerase Chain Reaction; RNA, Viral; Sensitivity and Specificity; Young Adult
Издатель
  • Elsevier B.V.
Тип документа
  • journal article
Тип лицензии Creative Commons
  • CC BY-NC-ND
Правовой статус документа
  • Свободная лицензия
Источник
  • scopus
COVID-19.рф: Информация против пандемии

Адрес: 115114, Москва, ул. Летниковская, д. 4, стр.5, офис 2.4
тел.\факс: +7(499)754-99-94, доб. 502
e-mail: covid19@neicon.ru

Проект реализуется с использованием гранта Президента Российской Федерации на развитие гражданского общества, предоставленного Фондом президентских грантов.

Содержимое сайта, если не указано иное, опубликовано в соответствии с лицензией Creative Commons «Attribution» («Атрибуция») 4.0 Всемирная (CC BY 4.0). Права на материалы, переданные партнерами проекта, принадлежат их правообладателям.

© 2025 NEICON