COVID-19.рф: ИНФОРМАЦИЯ ПРОТИВ ПАНДЕМИИ

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House Dust Mite Exposure Causes Increased Susceptibility of Nasal Epithelial Cells to Adenovirus Infection

M. Aydin, E. Naumova, F. Paulsen, W. Zhang, F. Gopon, C. Theis, S. Lutz, E. Ehrke-Schulz, W. Arnold, S. Wirth, A. Ehrhardt,
2020
https://doi.org/10.3390/v12101151

Adenovirus (AdV) infections in the respiratory tract may cause asthma exacerbation and allergic predisposition, and the house dust mite (HDM) may aggravate virus-induced asthma exacerbations. However, the underlying mechanisms of whether and how AdV affects asthmatic patients remains unclear. To address this question, we investigated nasal epithelial cells (NAEPCs) derived from a pediatric exacerbation study cohort for experimental analyses. We analyzed twenty-one different green-fluorescent protein- and luciferase-tagged AdV types in submerged 2D and organotypic 3D cell culture models. Transduction experiments revealed robust transduction of AdV type 5 (AdV5) in NAEPCs, which was associated with an increased uptake of AdV5 in the presence of HDM. In healthy and asthmatic NAEPCs exposed to HDM before infection, we observed a time- and dose-dependent increase of AdV5 uptake associated with upregulation of entry receptors for AdV5. Furthermore, electron microscopic and histologic analyses of 3D cell cultures revealed an impairment of the respiratory cilia after HDM exposition. This ex vivo pilot study shows the impact of AdV infection and HDM exposition in a primary cell culture model for asthma.

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Версии

  • 1. Version of Record от 2020-10-11

Метаданные

Об авторах
  • M. Aydin
    Children’s Hospital, Center for Clinical and Translational Research (CCTR), Helios University Medical Center Wuppertal, Witten/Herdecke University, 42283 Wuppertal, Germany;, malik.aydin@uni-wh.de, (M.A.);, stefan.wirth@uni-wh.de, (S.W.), Laboratory of Clinical Molecular Genetics & Epigenetics, Center for Biomedical Education and Research, School of Life Sciences (ZBAF), Faculty of Health, Witten/Herdecke University, 42283 Wuppertal, Germany
  • E. Naumova
    Department of Biological and Material Sciences in Dentistry, Faculty of Health, Witten/Herdecke University, 58455 Witten, Germany;, ella.naumova@uni-wh.de, (E.A.N.);, wolfgang.arnold@uni-wh.de, (W.H.A.)
  • F. Paulsen
    Institute of Functional and Clinical Anatomy, Friedrich Alexander University Erlangen-Nürnberg, 91054 Erlangen, Germany;, friedrich.paulsen@fau.de, Department of Topographic Anatomy and Operative Surgery, Sechenov University, 119146 Moscow, Russia
  • W. Zhang
    Institute of Virology and Microbiology, Center for Biomedical Education and Research (ZBAF), Department of Human Medicine, Faculty of Health, Witten/Herdecke University, 58453 Witten, Germany;, wenli.zhang@uni-wh.de, (W.Z.);, eric.ehrke-schulz@uni-wh.de, (E.E.-S.)
  • F. Gopon
    Clinics for Anesthesiology, Helios University Medical Center Wuppertal, Center for Clinical and Translational Research (CCTR), Witten/Herdecke University, 42283 Wuppertal, Germany;, felix.gopon@helios-gesundheit.de, (F.G.);, christian.theis@helios-gesundheit.de, (C.T.)
  • C. Theis
    Clinics for Anesthesiology, Helios University Medical Center Wuppertal, Center for Clinical and Translational Research (CCTR), Witten/Herdecke University, 42283 Wuppertal, Germany;, felix.gopon@helios-gesundheit.de, (F.G.);, christian.theis@helios-gesundheit.de, (C.T.)
  • S. Lutz
    Children’s Hospital, Helios Hospital Niederberg, Teaching Hospital of University Hospital Essen, 42549 Velbert, German;, soeren.lutz@helios-gesundheit.de
  • E. Ehrke-Schulz
    Institute of Virology and Microbiology, Center for Biomedical Education and Research (ZBAF), Department of Human Medicine, Faculty of Health, Witten/Herdecke University, 58453 Witten, Germany;, wenli.zhang@uni-wh.de, (W.Z.);, eric.ehrke-schulz@uni-wh.de, (E.E.-S.)
  • W. Arnold
    Department of Biological and Material Sciences in Dentistry, Faculty of Health, Witten/Herdecke University, 58455 Witten, Germany;, ella.naumova@uni-wh.de, (E.A.N.);, wolfgang.arnold@uni-wh.de, (W.H.A.)
  • S. Wirth
    Children’s Hospital, Center for Clinical and Translational Research (CCTR), Helios University Medical Center Wuppertal, Witten/Herdecke University, 42283 Wuppertal, Germany;, malik.aydin@uni-wh.de, (M.A.);, stefan.wirth@uni-wh.de, (S.W.)
  • A. Ehrhardt
    Institute of Virology and Microbiology, Center for Biomedical Education and Research (ZBAF), Department of Human Medicine, Faculty of Health, Witten/Herdecke University, 58453 Witten, Germany;, wenli.zhang@uni-wh.de, (W.Z.);, eric.ehrke-schulz@uni-wh.de, (E.E.-S.)
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  • Viruses
Том
  • 12
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  • MDPI
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  • journal article
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  • CC BY
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