Direct Visualization of Amlodipine Intervention into Living Cells by Means of Fluorescence Microscopy

C. Quentin; R. Gerasimaitė; A. Freidzon; L. Atabekyan; G. Lukinavičius; V. Belov; G. Mitronova

Статья

Direct Visualization of Amlodipine Intervention into Living Cells by Means of Fluorescence Microscopy

C. Quentin, R. Gerasimaitė, A. Freidzon, L. Atabekyan, G. Lukinavičius, V. Belov, G. Mitronova,

2021

https://doi.org/10.3390/molecules26102997

Amlodipine, a unique long-lasting calcium channel antagonist and antihypertensive drug, has weak fluorescence in aqueous solutions. In the current paper, we show that direct visualization of amlodipine in live cells is possible due to the enhanced emission in cellular environment. We examined the impact of pH, polarity and viscosity of the environment as well as protein binding on the spectral properties of amlodipine in vitro, and used quantum chemical calculations for assessing the mechanism of fluorescence quenching in aqueous solutions. The confocal fluorescence microscopy shows that the drug readily penetrates the plasma membrane and accumulates in the intracellular vesicles. Visible emission and photostability of amlodipine allow confocal time-lapse imaging and the drug uptake monitoring.

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1. Version of Record от 2021-05-18

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Об авторах

C. Quentin
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany;, christine.quentin@mpibpc.mpg.de, (C.Q.);, vladimir.belov@mpibpc.mpg.de, (V.N.B.)
R. Gerasimaitė
Chromatin Imaging and Labeling Group, Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany;, ruta.gerasimaite@mpibpc.mpg.de, (R.G.);, grazvydas.lukinavicius@mpibpc.mpg.de, (G.L.)
A. Freidzon
Federal Research Center Crystallography and Photonics, Photochemistry Center, Russian Academy of Sciences, Novatorov 7a, 119421 Moscow, Russia;, freidzon.sanya@gmail.com, (A.F.);, levat51@mail.ru, (L.S.A.), National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), Kashirskoye Shosse 31, 115409 Moscow, Russia
L. Atabekyan
Federal Research Center Crystallography and Photonics, Photochemistry Center, Russian Academy of Sciences, Novatorov 7a, 119421 Moscow, Russia;, freidzon.sanya@gmail.com, (A.F.);, levat51@mail.ru, (L.S.A.)
G. Lukinavičius
Chromatin Imaging and Labeling Group, Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany;, ruta.gerasimaite@mpibpc.mpg.de, (R.G.);, grazvydas.lukinavicius@mpibpc.mpg.de, (G.L.)
V. Belov
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany;, christine.quentin@mpibpc.mpg.de, (C.Q.);, vladimir.belov@mpibpc.mpg.de, (V.N.B.)
G. Mitronova
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany;, christine.quentin@mpibpc.mpg.de, (C.Q.);, vladimir.belov@mpibpc.mpg.de, (V.N.B.)

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Molecules

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2997

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MDPI

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journal article

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CC BY

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Статья

Direct Visualization of Amlodipine Intervention into Living Cells by Means of Fluorescence Microscopy

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