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Статья
Photonic toolbox for fast real-time polymerase chain reaction
2020
We present an optical scientist vision for a photonic platform for real-time polymerase chain reaction (qPCR) and reverse-transcription qPCR (qRT-PCR) that integrates a rapid-cycle fiber-optic PCR chamber with feedback-controlled laser heating, online fluorometric detection of PCR products, and all-optical thermometry using an optical readout from color centers of diamond nanoparticles. We show that, with a careful optimization of laser-induced heating and heat-removal geometry, this approach can radically shorten the qPCR thermal cycle relative to standard PCR systems, paving the way toward credit-card-size testing kits for fast point-of-care infection diagnosis. Faster photonic qPCR/qRT-PCR will enable a rapid and highly specific early detection of infections, including new viruses, such as SARS-CoV-2 at very early stages-in most cases, even before the onset of disease, providing much-needed lead time for early treatment and disease spread prevention.
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