Production technology and characteristics of the cell product obtained using the 'ESVIEF System' kit (developed by JoinTechCell LLC, Russian Federation) for isolation of a stromal vascular fraction from human adipose tissue are described. The use of subcutaneous fat as a source of stem and progenitor cells for regenerative medicine has become widespread during the last decade. The main advantage of using adipose tissue as a source of stem cells when compared with bone marrow is the lower invasiveness of the material sampling procedure during liposuction in addition to the significantly larger number of multipotent mesenchymal stromal cells obtained per unit of tissue volume. The development and implementation of devices for automation and standardisation of stem cell isolation from adipose tissue are important for the widespread use of stem cells in clinical practice. This work aimed to evaluate the effectiveness and safety of the cell production technology using the 'ESVIEF System' kit for isolation of a stromal vascular fraction from human adipose tissue. Adipose tissue samples obtained from patients during liposuction were used as clinical material. The obtained cell fractions were studied using microscopy, flow cytometry and cell culture methods. The viability of the stromal vascular fraction cells (nucleated) was 90.9 ± 0.3% with a total number of 0.81 ± 0.08 × 106 cells/ml of adipose tissue. The study showed that the 'ESVIEF System' kit that was developed for isolating stromal vascular fractions from human adipose tissue is a promising and safe technology for producing cell products.