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Статья
Thirty-thousand-year-old distant relative of giant icosahedral DNA viruses with a pandoravirus morphology
2021
The largest known DNA viruses infect Acanthamoeba and belong to two markedly different families. The Megaviridae exhibit pseudo-icosahedral virions up to 0.7 μm in diameter and adenine- thymine (AT)-rich genomes of up to 1.25 Mb encoding a thousand proteins. Like their Mimivirus prototype discovered 10 y ago, they entirely replicate within cytoplasmic virion factories. In contrast, the recently discovered Pandoraviruses exhibit larger amphora-shaped virions 1 μm in length and guanine-cytosine-rich genomes up to 2.8 Mb long encoding up to 2,500 proteins. Their replication involves the host nucleus. Whereas the Megaviridae share some general features with the previously described icosahedral large DNA viruses, the Pandoraviruses appear unrelated to them. Here we report the discovery of a third type of giant virus combining an even larger pandoraviruslike particle 1.5 μm in lengthwith a surprisingly smaller 600 kb AT-rich genome, a gene content more similar to Iridoviruses and Marseillevirus, and a fully cytoplasmic replication reminiscent of the Megaviridae. This suggests that pandoravirus-like particles may be associated with a variety of virus families more diverse than previously envisioned. This giant virus, named Pithovirus sibericum, was isolated from a
Цитирование
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Версии
- 1. Version of Record от 2021-04-27
Метаданные
Название журнала
- Proceedings of the National Academy of Sciences of the United States of America
Том
- 111
Выпуск
- 11
Страницы
- 4274-4279
Ключевые слова
- complementary DNA; proteome; Acanthamoeba; Acanthamoeba castellanii; amino acid sequence; article; cell nucleus; cell vacuole; cytoplasm; DNA virus; gene number; gene sequence; greenhouse effect; Iridovirus; lipid membrane; nonhuman; nucleotide sequence; permafrost; phylogeny; Pithovirus sibericum; priority journal; protein protein interaction; Smallpox virus; thawing; transmission electron microscopy; Upper Pleistocene; virion; virus genome; virus isolation; virus morphology; virus particle; virus replication; giant DNA virus; icosahedral capsid; late Pleistocene; Amoeba; Base Sequence; Cluster Analysis; Computational Biology; DNA Viruses; Gene Expression Profiling; Microscopy, Electron; Molecular Sequence Annotation; Molecular Sequence Data; Phylogeny; Proteomics; Sequence Analysis, DNA; Siberia; Soil Microbiology
Издатель
- National Academy of Sciences
Тип документа
- journal article
Источник
- scopus
