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Статья
Three-step procedure for preparation of pure Bacillus altitudinis ribonuclease
2021
Ribonucleases are considered as promising tools for anticancer treatment due to their selective cytotoxicity against tumor cells. We investigated a new RNase from Bacillus altitudinis termed BALNASE (B. altitudinisRNase). Balnase is a close homolog of the well-known cytotoxic binase, differing by only one amino acid residue: nonpolar hydrophobic alanine at position 106 in the balnase molecule is replaced by a polar uncharged threonine in binase. The most exciting question is how the physico-chemical properties and biological effects of RNase might be changed by A106T substitution. Here, we have developed a chromatography-based rapid and modern technique for the purification of this new RNase which allowed us to get a protein sample of high quality with specific activity of 1.2 × 106units in preparative amounts, suitable for further investigation of its biological properties. © 2015 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.
Цитирование
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Версии
- 1. Version of Record от 2021-04-27
Метаданные
Название журнала
- FEBS Open Bio
Том
- 6
Выпуск
- 1
Страницы
- 24-32
Ключевые слова
- alanine; balnase; ribonuclease; threonine; unclassified drug; Article; Bacillus; Bacillus altitudinis; bioinformatics; catalysis; enzyme activity; enzyme isolation; enzyme purification; hydrophobicity; immunoblotting; mass spectrometry; nonhuman; polyacrylamide gel electrophoresis; priority journal; zymography
Издатель
- Elsevier B.V.
Тип документа
- journal article
Тип лицензии Creative Commons
- CC
Правовой статус документа
- Свободная лицензия
Источник
- scopus
