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Статья
The native monomer of bacillus pumilus ribonuclease does not exist extracellularly
2021
Supported by crystallography studies, secreted ribonuclease of Bacillus pumilus (binase) has long been considered to be monomeric in form. Recent evidence obtained using native polyacrylamide gel electrophoresis and size-exclusion chromatography suggests that binase is in fact dimeric. To eliminate ambiguity and contradictions in the data we have measured conformational changes, hypochromic effect, and hydrodynamic radius of binase. The immutability of binase secondary structure upon transition from low to high protein concentration was registered, suggesting the binase dimerization immediately after translocation through the cell membrane and leading to detection of binase dimers only in the culture fluid regardless of ribonuclease concentration. Our results made it necessary to take a fresh look at the binase stability and cytotoxicity towards virus-infected or tumor cells. Copyright © 2018 Olga Ilinskaya et al.
Цитирование
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Версии
- 1. Version of Record от 2021-04-27
Метаданные
Название журнала
- BioMed Research International
Том
- 2018
Страницы
- -
Ключевые слова
- dimer; monomer; ribonuclease; Bacillus pumilus ribonuclease; ribonuclease; Article; Bacillus pumilus; bacterial membrane; bacterium culture; circular dichroism; concentration (parameters); conformational transition; diffusion; dimerization; hydrodynamics; nonhuman; polyacrylamide gel electrophoresis; protein conformation; protein secondary structure; ultraviolet spectroscopy; Bacillus pumilus; cell membrane; chemistry; enzymology; metabolism; protein domain; Bacillus pumilus; Cell Membrane; Protein Domains; Protein Structure, Secondary; Ribonucleases
Издатель
- Hindawi Limited
Тип документа
- journal article
Тип лицензии Creative Commons
- CC
Правовой статус документа
- Свободная лицензия
Источник
- scopus
