Статья

Ribonuclease from bacillus acts as an antiviral agent against negative- and positive-sense single stranded human respiratory RNA viruses

M. Shah, C. Müller, Y. Romanova, A. Mostafa, V. Ulyanova, S. Pleschka, O. Ilinskaya,
2021

Bacillus pumilus ribonuclease (binase) was shown to be a promising antiviral agent in animal models and cell cultures. However, the mode of its antiviral action remains unknown. To assess the binase effect on intracellular viral RNA we have selected single stranded negative- and positive-sense RNA viruses, influenza virus, and rhinovirus, respectively, which annually cause respiratory illnesses and are characterized by high contagious nature, mutation rate, and antigen variability. We have shown that binase exerts an antiviral effect on both viruses at the same concentration, which does not alter the spectrum of A549 cellular proteins and expression of housekeeping genes. The titers of influenza A (H1N1pdm) virus and human rhinovirus serotype 1A were reduced by 40% and 65%, respectively. A preincubation of influenza virus with binase before infection significantly reduced viral titer after single-cycle replication of the virus. Using influenza A virus mini genome system we showed that binase reduced GFP reporter signaling indicating a binase action on the expression of viral mRNA. Binase reduced the level of H1N1pdm viral NP mRNA accumulation in A549 cells by 20%. Since the viral mRNA is a possible target for binase this agent could be potentially applied in the antiviral therapy against both negative- and positive-sense RNA viruses. Copyright © 2017 Raihan Shah Mahmud et al.

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  • 1. Version of Record от 2021-04-27

Метаданные

Об авторах
  • M. Shah
    Institute of Fundamental Medicine and Biology, Kazan Federal University, Kremlevskaya Street 18, Kazan, 420008, Russian Federation
  • C. Müller
    Institute of Medical Virology, Justus Liebig University, Schubert Street 81, Giessen, 35392, Germany
  • Y. Romanova
    Center of Scientific Excellence for Influenza Viruses, National Research Centre (NRC), El-Buhouth Street 87, Dokki, Cairo, 12311, Egypt
  • A. Mostafa
  • V. Ulyanova
  • S. Pleschka
  • O. Ilinskaya
Название журнала
  • BioMed Research International
Том
  • 2017
Страницы
  • -
Ключевые слова
  • antivirus agent; bacterial enzyme; binase; cell protein; ribonuclease; unclassified drug; virus RNA; antivirus agent; Bacillus pumilus ribonuclease; ribonuclease; A-549 cell line; animal cell; antiviral activity; antiviral therapy; Article; Bacillus pumilus; cell viability; controlled study; enzyme activity; female; gene expression; HeLa cell line; housekeeping gene; human; human cell; Human rhinovirus A1; Influenza A virus (H1N1); Influenza virus; MDCK-II cell line; negative-strand RNA virus; nonhuman; positive-strand RNA virus; protein expression; real time polymerase chain reaction; Rhinovirus; virus replication; virus titration; Bacillus; chemistry; drug effects; enzymology; genetics; pathogenicity; Rhinovirus; RNA virus; A549 Cells; Antiviral Agents; Bacillus; Humans; Influenza A Virus, H1N1 Subtype; Rhinovirus; Ribonucleases; RNA Viruses; RNA, Viral; Virus Replication
Издатель
  • Hindawi Limited
Тип документа
  • journal article
Тип лицензии Creative Commons
  • CC
Правовой статус документа
  • Свободная лицензия
Источник
  • scopus