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Статья
Humoral and cellular immune responses to Yersinia pestis Pla antigen in humans immunized with live plague vaccine
2021
Background: To establish correlates of human immunity to the live plague vaccine (LPV), we analyzed parameters of cellular and antibody response to the plasminogen activator Pla of Y. pestis. This outer membrane protease is an essential virulence factor that is steadily expressed by Y. pestis. Methodology/Principal findings: PBMCs and sera were obtained from a cohort of naïve (n = 17) and LPV-vaccinated (n = 34) donors. Anti-Pla antibodies of different classes and IgG subclasses were determined by ELISA and immunoblotting. The analysis of antibody response was complicated with a strong reactivity of Pla with normal human sera. The linear Pla B-cell epitopes were mapped using a library of 15-mer overlapping peptides. Twelve peptides that reacted specifically with sera of vaccinated donors were found together with a major cross-reacting peptide IPNISPDSFTVAAST located at the N-terminus. PBMCs were stimulated with recombinant Pla followed by proliferative analysis and cytokine profiling. The T-cell recall response was pronounced in vaccinees less than a year post-immunization, and became Th17-polarized over time after many rounds of vaccination. Conclusions/Significance: The Pla protein can serve as a biomarker of successful vaccination with LPV. The diagnostic use of Pla will require elimination of cross-reactive parts of the antigen. © 2018 Feodorova et al. http://creativecommons.org/licenses/by/4.0/
Цитирование
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Версии
- 1. Version of Record от 2021-04-27
Метаданные
Название журнала
- PLoS Neglected Tropical Diseases
Том
- 12
Выпуск
- 6
Страницы
- -
Ключевые слова
- concanavalin A; gamma interferon; immunoglobulin A; immunoglobulin E; immunoglobulin G; immunoglobulin G1; immunoglobulin G2; immunoglobulin G3; immunoglobulin G4; immunoglobulin M; interleukin 10; interleukin 17; interleukin 4; plague vaccine; plasminogen activator; plasminogen activator antibody; protein antibody; recombinant plasminogen activator; synthetic peptide; tumor necrosis factor; unclassified drug; bacterial antigen; bacterial protein; biological marker; cytokine; epitope; immunoglobulin G; live vaccine; Pla protease, Yersinia pestis; plague vaccine; plasminogen activator; virulence factor; adult; aged; amino acid sequence; amino terminal sequence; antibody response; Article; B lymphocyte; bacterial immunity; bacterial virulence; cell polarity; cellular immunity; clinical article; cohort analysis; controlled study; correlation coefficient; cross reaction; cytokine production; donor; enzyme linked immunosorbent assay; epitope mapping; gene expression; human; human cell; humoral immunity; immunoblotting; immunoglobulin blood level; immunoreactivity; lymphocyte proliferation; nonhuman; normal human; peptide library; peripheral blood mononuclear cell; plague; serum; T lymphocyte; Th17 cell; vaccination; Yersinia pestis; biosynthesis; blood; female; immunology; male; microbiology; middle aged; plague; vaccination; Yersinia pestis; Adult; Aged; Antigens, Bacterial; Bacterial Proteins; Biomarkers; Cytokines; Epitopes, B-Lymphocyte; Female; Humans; Immunity, Cellular; Immunity, Humoral; Immunoglobulin G; Male; Middle Aged; Plague; Plague Vaccine; Plasminogen Activators; Th17 Cells; Vaccination; Vaccines, Live, Unattenuated; Virulence Factors; Yersinia pestis
Издатель
- Public Library of Science
Тип документа
- journal article
Тип лицензии Creative Commons
- CC
Правовой статус документа
- Свободная лицензия
Источник
- scopus
