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Статья
Proteomics of diphtheria toxoid vaccines reveals multiple proteins that are immunogenic and may contribute to protection of humans against Corynebacterium diphtheriae
2021
Introduced for mass immunization in the 1920s, vaccines against diphtheria are among the oldest and safest vaccines known. The basic principle of their production is the inactivation of purified diphtheria toxin by formaldehyde cross-linking, which converts the potentially fatal toxin in a completely harmless protein aggregate, which is still immunogenic. Since in addition to diphtheria toxin also other proteins may be secreted by Corynebacterium diphtheriae during cultivation, we assumed that diphtheria toxoid might not be the only component present in the vaccine. To address this question, we established a protocol to reverse formaldehyde cross-linking and carried out mass spectrometric analyses. Different secreted, membrane-associated and cytoplasmic proteins of C. diphtheriae were detected in several vaccine preparations from across the world. Based on these results, bioinformatics and Western blot analyses were applied to characterize if these proteins are immunogenic and may therefore support protection against C. diphtheriae. In frame of this study, we could show that the C. diphtheriae toxoid vaccines induce antibodies against different C. diphtheriae proteins and against diphtheria toxin secreted by Corynebacterium ulcerans, an emerging pathogen which is outnumbering C. diphtheriae as cause of diphtheria-like illness in Western Europe. © 2019
Цитирование
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Версии
- 1. Version of Record от 2021-04-27
Метаданные
Название журнала
- Vaccine
Том
- 37
Выпуск
- 23
Страницы
- 3061-3070
Ключевые слова
- bacterial protein; diphtheria pertussis poliomyelitis tetanus Haemophilus influenzae type b vaccine; diphtheria toxin; diphtheria vaccine; formaldehyde; bacterial protein; bacterium antibody; cross linking reagent; diphtheria toxoid; pertussis vaccine; adult; antibody production; Article; bacterial growth; bioinformatics; clinical article; controlled study; Corynebacterium diphtheriae; Corynebacterium ulcerans; cross linking; diphtheria; hematopoietic stem cell transplantation; human; immunogenicity; mass spectrometry; nonhuman; prediction; priority journal; proteomics; revaccination; Western blotting; chemistry; Corynebacterium; Corynebacterium diphtheriae; cytoplasm; diphtheria; Europe; immunology; proteomics; vaccination; Antibodies, Bacterial; Bacterial Proteins; Corynebacterium; Corynebacterium diphtheriae; Cross-Linking Reagents; Cytoplasm; Diphtheria; Diphtheria Toxoid; Europe; Formaldehyde; Humans; Mass Spectrometry; Pertussis Vaccine; Proteomics; Vaccination
Издатель
- Elsevier Ltd
Тип документа
- journal article
Тип лицензии Creative Commons
- CC
Правовой статус документа
- Свободная лицензия
Источник
- scopus
