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Статья
Two live attenuated vaccines against recent low–and highly pathogenic H7N9 influenza viruses are safe and immunogenic in ferrets
2021
Influenza H7N9 virus is a potentially pandemic subtype to which most people are immunologically naïve. To be better prepared for the potential occurrence of an H7N9 pandemic, in 2017 the World Health Organization recommended developing candidate vaccine viruses from two new H7N9 viruses, A/Guangdong/17SF003/2016 (A/GD) and A/Hong Kong/125/2017 (A/HK). This report describes the development of live attenuated influenza vaccine (LAIV) candidates against A/GD and A/HK viruses and study of their safety and immunogenicity in the ferret model in order to choose the most promising one for a phase I clinical trial. The A/HK-based vaccine candidate (A/17/HK) was developed by classical reassortment in eggs. The A/GD-based vaccine candidate (A/17/GD) was generated by reverse genetics. Ferrets were vaccinated with two doses of LAIV or phosphate-buffered saline. Both H7N9 LAIVs tested were safe for ferrets, as shown by absence of clinical signs, and by virological and histological data; they were immunogenic after a single vaccination. These results provide a compelling argument for further testing of these vaccines in volunteers. Since the A/HK virus represents the cluster that has caused the majority of human cases, and because the A/HK-based LAIV candidate was developed by classical reassortment, this is the preferred candidate for a phase I clinical trial. © 2018 by the World Health Organization.Licensee MDPI, Basel, Switzerland.
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Версии
- 1. Version of Record от 2021-04-27
Метаданные
Название журнала
- Vaccines
Том
- 6
Выпуск
- 4
Страницы
- -
Ключевые слова
- immunoglobulin A; immunoglobulin G; influenza vaccine; animal experiment; animal model; animal tissue; Article; autopsy; body weight; clinical feature; controlled study; enzyme linked immunosorbent assay; genetic reassortment; hemagglutination; histopathology; Influenza A virus (H7N9); male; morbidity; Mustela putorius furo; nonhuman; phenotype; randomized controlled trial; real time polymerase chain reaction; revaccination; reverse genetics; vaccination; vaccine immunogenicity; virus detection; virus load; virus replication
Издатель
- MDPI AG
Тип документа
- journal article
Тип лицензии Creative Commons
- CC
Правовой статус документа
- Свободная лицензия
Источник
- scopus
