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Статья
Development of qPCR platform with probes for quantifying prevalent and biomedically relevant human gut microbial taxa
2021
Nowadays the advent of innovative high-throughput sequencing allows obtaining high-quality microbiome profiling. However, PCR-based tests are still considered the “golden standard” for many clinical applications. Here, we designed a qPCR-based platform with fluorescent-labeled oligonucleotide probes for assessing human gut microbiome composition. The system allows conducting qualitative and semiquantitative analysis for 12 prokaryotic taxa that are prevalent in the human gut and associated with diseases, diet, age and other factors. The platform was validated by comparing microbiome profile data obtained with two different methods - the platform and high-throughput 16S rRNA sequencing - across 42 stool samples. The test can form the basis for precise and cost-efficient microbiome assay for large-scale surveys including clinical trials with interventions related to diet and disease risks. © 2020 Elsevier Ltd
Цитирование
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Версии
- 1. Version of Record от 2021-04-27
Метаданные
Название журнала
- Molecular and Cellular Probes
Том
- 52
Страницы
- -
Ключевые слова
- DNA fragment; plasmid DNA; RNA 16S; age; Akkermansia; amplicon; Article; Bacteroides; Bifidobacterium; Blautia; Christensenellaceae; comparative study; consensus sequence; controlled study; diseases; DNA extraction; Enterobacteriaceae; Enterococcus; high throughput sequencing; human; inflammatory bowel disease; intestine flora; Lactobacillus; Methanobrevibacter; non insulin dependent diabetes mellitus; nonhuman; nucleotide sequence; oligonucleotide probe; Prevotella; priority journal; real time polymerase chain reaction; recombinant plasmid; Ruminococcus; spectrophotometry; Streptococcus; taxon
Издатель
- Academic Press
Тип документа
- journal article
Тип лицензии Creative Commons
- CC
Правовой статус документа
- Свободная лицензия
Источник
- scopus
