Статья

COVID-19 biobank: features of the cytokine profile [Биобанк COVID-19: особенности цитокинового профиля]

N. Sushentseva, O. Popov, S. Apalko, A. Anisenkova, S. Azarenko, K. Smantserev, D. Khobotnikov, T. Gladysheva, E. Minina, S. Strelyukhina, S. Urazov, D. Pavlovich, S. Fridman, S. Shcherbak,
2021

Aim. Using a collection of samples from the biobank of City Hospital № 40 of St. Petersburg, to study the cytokine profile in patients with coronavirus disease 2019 (COVID-19) and sepsis, in comparison with patients with abdominal inflammation and septicemia. Material and methods. The study included serum samples from 181 patients with sepsis and COVID-19 (127 patients with a diagnosis confirmed by polymerase chain reaction (PCR); 54 patients with a negative PCR test, but with a characteristic computed tomographic lung performance) and 47 patients with abdominal sepsis. The content of cytokines was determined using a multiplex immunofluorescence analysis based on the Luminex xMAP technology using the HCYTOMAG-60K panel — a soluble CD40 ligand (sCD40L), interleukin-1α (IL-1α), interleukin-1β (IL-1β), interleukin 6 (IL-6), interleukin 8 (IL-8), monocyte chemoattractant protein 1 (MCP-1), tumor necrosis factor alpha (TNFα), vascular endothelial growth factor (VEGF). Other laboratory parameters (C-reactive protein (CRP), ferritin, procalcitonin) were taken from patient records. Normality of distribution was assessed by the Shapiro-Wilk test. To compare groups, the Mann-Whitney test for independent samples, Wilcoxon test for dependent samples, and the Kruskal-Wallis test with Bonferroni correction for multiple comparisons were used. Results. In patients with sepsis and COVID-19 infection, no differences in the concentrations of cytokines, ferritin and CRP were found between the groups with detected and not detected virus by PCR test. Based on this, this group was considered homogeneous when studying the cytokine profile. It was shown that in patients with sepsis and COVID-19, significantly higher levels of sCD40L (p

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Версии

  • 1. Version of Record от 2021-04-27

Метаданные

Об авторах
  • N. Sushentseva
    City Hospital, № 40, Sestroretsk, Russian Federation
  • O. Popov
    Saint Petersburg State University, Saint-Petersburg, Russian Federation
  • S. Apalko
  • A. Anisenkova
  • S. Azarenko
  • K. Smantserev
  • D. Khobotnikov
  • T. Gladysheva
  • E. Minina
  • S. Strelyukhina
  • S. Urazov
  • D. Pavlovich
  • S. Fridman
  • S. Shcherbak
Название журнала
  • Cardiovascular Therapy and Prevention (Russian Federation)
Том
  • 19
Выпуск
  • 6
Страницы
  • -
Ключевые слова
  • C reactive protein; CD40 ligand; ferritin; interleukin 17; interleukin 1alpha; interleukin 1beta; interleukin 6; interleukin 8; monocyte chemotactic protein 1; procalcitonin; tumor necrosis factor; vasculotropin; adult; adult respiratory distress syndrome; Article; biobank; controlled study; coronary artery disease; coronavirus disease 2019; endothelium injury; enzyme linked immunosorbent assay; female; human; human tissue; immunofluorescence; Kruskal Wallis test; major clinical study; male; medical record; nonhuman; polymerase chain reaction; protein expression; rank sum test; SARS coronavirus; sepsis; virus
Издатель
  • Vserossiiskoe Obshchestvo Kardiologov
Тип документа
  • journal article
Тип лицензии Creative Commons
  • CC
Правовой статус документа
  • Свободная лицензия
Источник
  • scopus