Поиск по репозиторию
Статья
Impact of pathogen reduction methods on immunological properties of the COVID-19 convalescent plasma
2020
ABSTRACT Background and Objectives COVID-19 convalescent plasma has become an experimental treatment option against SARS-CoV2. The aim of this study is to assess the impact of different pathogen reduction methods on the immunological properties of COVID-19 convalescent plasma. Materials and Methods A total of 140 plasma doses collected by plasmapheresis from COVID-19 convalescent donors were subjected to pathogen reduction by three different methods: methylene blue (M), riboflavin (R), and amotosalen (A). To conduct a paired two-sample comparison, individual plasma doses were divided into 2 samples that were subjected to one of these methods. The titres of SARS-CoV2 neutralizing antibodies (NtAbs) and levels of specific immunoglobulins to RBD, S- and N-proteins of SARS-CoV-2 were measured before and after pathogen reduction. Results All methods reduced NtAbs titres significantly but not equally: among units with the initial titre 80 or above, 81% of units had unchanged titres while 19% decreased by 1 step after methylene blue; 60% were unchanged and 40% decreased by 1 step after amotosalen; whereas after riboflavin 43% were unchanged, 50% had a one-step decrease and 7% a two-step decrease. Paired two-sample comparisons (M vs A, M vs R and A vs R) revealed that the most prominent and statistically significant decrease in all studied parameters (except anti-RBD) resulted from the riboflavin treatment. Conclusion Pathogen reduction with methylene blue and amotosalen provides the greater likelihood of preserving the immunological properties of the COVID-19 convalescent plasma compared to riboflavin.
Цитирование
