Статья

Using experimental ex vivo models to develop COVID-19 pathogenetic therapy and complications prevention agents

D. Laptev, S. Petunov, O. Nechaykina, D. Bobkov, A. Radilov,
2020

COVID-19 is a disease characterized by damage to the lower respiratory tract, development of the acute respiratory distress syndrome, in severe cases — multiple organ failure, including acute heart failure and cardiomyopathy. This study aimed to evaluate the effectiveness of the developed COVID-19 pathogenetic therapy and complications prevention agents using the ex vivo isolated lung and heart models. Isolated organs of white rats were used for the research; the dynamics of functional indicators were analyzed. An amino acid-peptide complex (APC) from a thermally treated milk protein hydrolyzate was used as the experimental COVID-19 pathogenetic therapy and complications prevention agent. Introduction of the APC to the isolated cardiopulmonary complex perfusate slowed down development of pulmonary edema in the experimental group; the organ's weight was 1.5 times less than in the control group (p = 0.0158). We have also registered an airway resistance downtrend. APC supported contractile activity of the isolated myocardium suffering ischemia-reperfusion: the growth of the left ventricular end diastolic pressure was 34% smaller than that registered in the control group (p < 0.05). The APC's cardioprotective effect relies on the endothelium-dependent mechanisms. The ex vivo method is highly informative. It allows assessing reactivity of the isolated organs exposed to biologically active substances and determining the possibilities of compensating for functional changes.

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Версии

  • 1. Version of Record от 2020-12-12

Метаданные

Об авторах
  • D. Laptev
    Research Institute of Hygiene, Occupational Pathology and Human Ecology FMBA, Leningrad region, Russia
  • S. Petunov
    Research Institute of Hygiene, Occupational Pathology and Human Ecology FMBA, Leningrad region, Russia
  • O. Nechaykina
    Research Institute of Hygiene, Occupational Pathology and Human Ecology FMBA, Leningrad region, Russia
  • D. Bobkov
    Research Institute of Hygiene, Occupational Pathology and Human Ecology FMBA, Leningrad region, Russia
  • A. Radilov
    Research Institute of Hygiene, Occupational Pathology and Human Ecology FMBA, Leningrad region, Russia
Название журнала
  • Bulletin officiel
Выпуск
  • (4)2020
Издатель
  • Federal Medical Biological Agency
Тип документа
  • journal article
Тип лицензии Creative Commons
  • CC BY
Правовой статус документа
  • Свободная лицензия
Источник
  • dimensions