Polymer Particles Bearing Recombinant LEL CD81 as Trapping Systems for Hepatitis C Virus

D. Polyakov; E. Sinitsyna; N. Grudinina; M. Antipchik; R. Sakhabeev; V. Korzhikov-Vlakh; M. Shavlovsky; E. Korzhikova-Vlakh; T. Tennikova

Статья

Polymer Particles Bearing Recombinant LEL CD81 as Trapping Systems for Hepatitis C Virus

D. Polyakov, E. Sinitsyna, N. Grudinina, M. Antipchik, R. Sakhabeev, V. Korzhikov-Vlakh, M. Shavlovsky, E. Korzhikova-Vlakh, T. Tennikova,

2021

https://doi.org/10.3390/pharmaceutics13050672

Hepatitis C is one of the most common social diseases in the world. The improvements in both the early diagnostics of the hepatitis C and the treatment of acute viremia caused by hepatitis C virus are undoubtedly an urgent task. In present work, we offered the micro- and nanotraps for the capturing of HCV. As a capturing moiety, we designed and synthesized in E. coli a fusion protein consisting of large extracellular loop of CD81 receptor and streptavidin as spacing part. The obtained protein has been immobilized on the surface of PLA-based micro- and nanoparticles. The developed trapping systems were characterized in terms of their physico-chemical properties. In order to illustrate the ability of developed micro- and nanotraps to bind HCV, E2 core protein of HCV was synthesized as a fusion protein with GFP. Interaction of E2 protein and hepatitis C virus-mimicking particles with the developed trapping systems were testified by several methods.

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1. Version of Record от 2021-05-07

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D. Polyakov
Institute of Experimental Medicine, Acad. Pavlov Street 12, 197376 St. Petersburg, Russia;, ravendoctor@mail.ru, (D.P.);, strangecatnap@gmail.com, (N.G.);, helm505@mail.ru, (R.S.);, mmsch@rambler.ru, (M.S.)
E. Sinitsyna
Institute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoy pr. 31, 199004 St. Petersburg, Russia;, kat_sinitsyna@mail.ru, (E.S.);, volokitinamariya@yandex.ru, (M.A.), Institute of Chemistry, Saint-Petersburg State University, Universitetsky pr. 26, 198504 St. Petersburg, Russia;, v.korzhikov-vlakh@spbu.ru
N. Grudinina
Institute of Experimental Medicine, Acad. Pavlov Street 12, 197376 St. Petersburg, Russia;, ravendoctor@mail.ru, (D.P.);, strangecatnap@gmail.com, (N.G.);, helm505@mail.ru, (R.S.);, mmsch@rambler.ru, (M.S.)
M. Antipchik
Institute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoy pr. 31, 199004 St. Petersburg, Russia;, kat_sinitsyna@mail.ru, (E.S.);, volokitinamariya@yandex.ru, (M.A.)
R. Sakhabeev
Institute of Experimental Medicine, Acad. Pavlov Street 12, 197376 St. Petersburg, Russia;, ravendoctor@mail.ru, (D.P.);, strangecatnap@gmail.com, (N.G.);, helm505@mail.ru, (R.S.);, mmsch@rambler.ru, (M.S.)
V. Korzhikov-Vlakh
Institute of Chemistry, Saint-Petersburg State University, Universitetsky pr. 26, 198504 St. Petersburg, Russia;, v.korzhikov-vlakh@spbu.ru
M. Shavlovsky
Institute of Experimental Medicine, Acad. Pavlov Street 12, 197376 St. Petersburg, Russia;, ravendoctor@mail.ru, (D.P.);, strangecatnap@gmail.com, (N.G.);, helm505@mail.ru, (R.S.);, mmsch@rambler.ru, (M.S.)
E. Korzhikova-Vlakh
Institute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoy pr. 31, 199004 St. Petersburg, Russia;, kat_sinitsyna@mail.ru, (E.S.);, volokitinamariya@yandex.ru, (M.A.), Institute of Chemistry, Saint-Petersburg State University, Universitetsky pr. 26, 198504 St. Petersburg, Russia;, v.korzhikov-vlakh@spbu.ru
T. Tennikova
Institute of Chemistry, Saint-Petersburg State University, Universitetsky pr. 26, 198504 St. Petersburg, Russia;, v.korzhikov-vlakh@spbu.ru

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Polymer Particles Bearing Recombinant LEL CD81 as Trapping Systems for Hepatitis C Virus

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