Поиск по репозиторию
Статья
Optimization of electrofusion parameters for producing hybridomas synthesizing human monoclonal antibodies
2021
Monoclonal antibodies are an established treatment for many diseases, including cancer, toxic conditions and infectious diseases. The goal of this work was to optimize the conditions for obtaining hybridomas secreting human monoclonal IgG antibodies. A new protocol was developed for efficient electrofusion of human B lymphocytes with partner cells. Electrofusion parameters were optimized, and the preferred partner cell line and the ratio of cells involved in the fusion were selected. Two myeloma cell lines, K6H6/B5 and SHM-D33, were tested in detail, and the highest fusion efficiency was observed for K6H6/B5. Three hybridomas that secreted fully human monoclonal IgG antibodies were obtained using an optimized protocol electrofusion; the secretion was observed within a month, which indicates the stability of the clones and the absence of chromosome segregation.
Цитирование
Похожие публикации
Версии
- 1. Version of Record от 2021-01-01
Метаданные
Название журнала
- Biotekhnologiya
Том
- 37
Выпуск
- 2
Страницы
- 65-75
Финансирующая организация
- Ministry of Education and Science of the Russian Federation
Номер гранта
- 075‑15‑2019‑1671
Тип документа
- journal article
Тип лицензии Creative Commons
- CC BY
Правовой статус документа
- Свободная лицензия
Источник
- scopus
