Статья

Immunoinformatics analysis to design novel epitope based vaccine candidate targeting the glycoprotein and nucleoprotein of Lassa mammarenavirus (LASMV) using strains from Nigeria

O. Abass, V. Timofeev, B. Sarkar, D. Onobun, S. Ogunsola, A. Aiyenuro, A. Aborode, A. Aigboje, B. Omobolanle, A. Imolele, A. Abiodun,
2021

Lassa mammarenavirus (LASMV) is responsible for a specific type of acute viral hemorrhagic fever known as Lassa fever. Lack of effective treatments and counter-measures against the virus has resulted in a high mortality rate in its endemic regions. Therefore, in this study, a novel epitope-based vaccine has been designed using the methods of immunoinformatics targeting the glycoprotein and nucleoprotein of the virus. After numerous robust analyses, two CTL epitopes, eight HTL epitopes and seven B-cell epitopes were finally selected for constructing the vaccine. All these most promising epitopes were found to be antigenic, non-allergenic, nontoxic and non-human homolog, which made them suitable for designing the subunit vaccine. Furthermore, the selected T-cell epitopes which were found to be fully conserved across different isolates of the virus, were also considered for final vaccine construction. After that, numerous validation experiments, i.e. molecular docking, molecular dynamics simulation and immune simulation were conducted, which predicted that our designed vaccine should be stable within the biological environment and effective in combating the LASMV infection. In the end, codon adaptation and in silico cloning studies were performed to design a recombinant plasmid for producing the vaccine industrially. However, further in vitro and in vivo assessments should be done on the constructed vaccine to finally confirm its safety and efficacy. Communicated by Ramaswamy H. Sarma.

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Версии

  • 1. Version of Record от 2021-01-01

Метаданные

Об авторах
  • O. Abass
    Centre for BioCode, Ambrose Alli University
  • V. Timofeev
    Shubnikov Institute of Crystallography, Russian Academy of Sciences
  • B. Sarkar
    Jahangirnagar University
  • D. Onobun
    Centre for BioCode, Ambrose Alli University
  • S. Ogunsola
    Federal University of Technology, Akure, University of Manitoba
  • A. Aiyenuro
    University of Cambridge
  • A. Aborode
    Research and Development, Research and Development
  • A. Aigboje
    Ambrose Alli University
  • B. Omobolanle
    University of Ilorin
  • A. Imolele
    Ambrose Alli University
  • A. Abiodun
    Molecular Biology & Simulations (Mols & Sims) Centre
Название журнала
  • Journal of Biomolecular Structure and Dynamics
Финансирующая организация
  • Ministerstwo Nauki i Szkolnictwa Wyższego
Номер гранта
  • undefined
Тип документа
  • journal article
Тип лицензии Creative Commons
  • CC BY
Правовой статус документа
  • Свободная лицензия
Источник
  • scopus