Статья

Application of real-time PCR to significantly reduce the time to obtain recombinant MVA virus

O. Orlova, D. Glazkova, G. Tsyganova, I. Antoshkina, R. Mintaev, A. Tikhonov, E. Bogoslovskaya, G. Shipulin,
2021

Obtaining a pure recombinant Modified Vaccinia Ankara (MVA) virus is a multistage, time-consuming procedure. We describe a novel single-tube real-time PCR which enables determination of the amount of wild type and recombinant viruses and their ratio in plaques. Use of the real-time PCR significantly reduce the time and efforts needed to obtain purified recombinant MVA. The new approach has been applied to generate recombinant MVAs encoding different SARS-COV-2 antigens.

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  • 1. Version of Record от 2021-03-01

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Об авторах
  • O. Orlova
    Centre for Strategic Planning and Management of Biomedical Health Risks
  • D. Glazkova
    Centre for Strategic Planning and Management of Biomedical Health Risks
  • G. Tsyganova
    Centre for Strategic Planning and Management of Biomedical Health Risks
  • I. Antoshkina
    Centre for Strategic Planning and Management of Biomedical Health Risks
  • R. Mintaev
    Centre for Strategic Planning and Management of Biomedical Health Risks, I. I. Mechnikov Research Institute for Vaccines and Sera RAMS
  • A. Tikhonov
    Centre for Strategic Planning and Management of Biomedical Health Risks
  • E. Bogoslovskaya
    Centre for Strategic Planning and Management of Biomedical Health Risks
  • G. Shipulin
    Centre for Strategic Planning and Management of Biomedical Health Risks
Название журнала
  • Journal of Virological Methods
Том
  • 289
Финансирующая организация
  • Russian Foundation for Basic Research
Номер гранта
  • 20-04-60100
Тип документа
  • journal article
Тип лицензии Creative Commons
  • CC BY
Правовой статус документа
  • Свободная лицензия
Источник
  • scopus