Introduction. The article presents research materials on the impact of the COVID-19 pandemic on labor activity, conducted during the period of active development of the epidemic spread around the world. The aim of study is to analyze the labor activity of employees during the pandemic, taking into account psychophysiological indicators. Materials and methods. Comparative analysis of questionnaire data (used the questionnaire developed by the teachers of the Department "Management of safety in technosphere" Russian University of transport) railway workers during a pandemic COVID-19, considered the issues of psychological state, but also studied a stress level of employees and predicting the impact of a pandemia on the state of mental health of railway workers. 50 female guides took part in the survey, most of them were women (about 70%) with continuous work experience in this profession. Results. Guides generally adequately assess the health risk of COVID-19 exposure. However, there are several people who "underestimate the risk of exposure to the factor" (about 20%), and their number has increased during the period of lifting the quarantine. Comparative analysis showed that during the quarantine period, employees felt psychoemotional stress, increased the number of anxieties, depression, and nervous disorders. The obtained data confirm the expediency of further research in this direction, the need to normalize the work of employees in the context of the development of the pandemia and the preservation of their health. Conclusions. It is necessary to wait and make every effort to fight COVID-19. It is also necessary to control the psychological state, if you ignore the fear of employees that they may become infected, then this may open up diseases of a different nature.