Diseases of animals and humans are largely environmentally related. With the timely detection of the disease, it is possible to avoid severe consequences, both for human health, animals, and the environment as a whole. The article describes the process of creating diagnostic test systems, including obtaining used nanoparticles and describing their physicochemical properties as the kinetics of gold and silver nanoparticles in mono-and binary hydrosols using spectroscopic methods of investigation, determined the size of silver nanoparticles on the basis of the method of cross-correlation spectroscopy of photons and transmission electron microscopy and the effect of ultrasound on the spectroscopic data hydrosols of gold and silver. The article contains a description of the technology of obtaining bionanoconjugates – a key link for creating diagnostic test systems. The results of the development of diagnosticums are presented at the end of the article and the corresponding conclusions are drawn. © 2018, Kaunas University of Technology. All rights reserved.