Статья

Effect of hemagglutinin glycosylation on influenza virus susceptibility to neuraminidase inhibitors

V. Mishin, D. Novikov, F. Hayden, L. Gubareva,
2021

Inhibition of neuraminidase (NA) activity prevents release of progeny virions from influenza-infected cells and removal of neuraminic (sialic) acid moieties from glycans attached to hemagglutinin (HA). Neuraminic acid moieties situated near the HA receptor-binding site can reduce the efficiency of virus binding and decrease viral dependence on NA activity for replication. With the use of reverse genetics technique, we investigated the effect of glycans attached at Asn 94a, 129, and 163 on the virus susceptibility to NA inhibitors in MDCK cells and demonstrated that the glycan attached at Asn 163 plays a dominant role in compensation for the loss of NA activity. Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Цитирование

Похожие публикации

Источник

Версии

  • 1. Version of Record от 2021-04-27

Метаданные

Об авторах
  • V. Mishin
    Department of Internal Medicine, School of Medicine, University of Virginia, Charlottesville, VA, United States
  • D. Novikov
    Department of Pathology, School of Medicine, University of Virginia, Charlottesville, VA, United States
  • F. Hayden
    Jordan Hall, 1300 Jefferson Park Ave., Charlottesville, VA 22908, United States
  • L. Gubareva
    Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN, United States
Название журнала
  • Journal of Virology
Том
  • 79
Выпуск
  • 19
Страницы
  • 12416-12424
Ключевые слова
  • asparagine; glycan derivative; hemagglutinin; sialic acid; sialidase; sialidase inhibitor; animal cell; article; cell strain; controlled study; enzyme activity; glycosylation; Influenza virus; nonhuman; priority journal; receptor binding; virion; virus replication; Acetamides; Agglutination; Animals; Antiviral Agents; Cell Line; Dogs; Enzyme Inhibitors; Erythrocytes; Glycosylation; Guanidines; Hemagglutinins; Models, Molecular; Neuraminidase; Orthomyxoviridae; Oseltamivir; Pyrans; Sialic Acids; Zanamivir; Influenza virus
Тип документа
  • journal article
Источник
  • scopus