Статья

New personal sampler for viable airborne viruses: Feasibility study

I. Agranovski, A. Safatov, A. Borodulin, O. Pyankov, V. Petrishchenko, A. Sergeev, A. Sergeev, V. Agranovski, S. Grinshpun,
2021

While various sampling methods exist for collecting and enumerating airborne bacteria and fungi, no credible methodology has yet been developed for airborne viruses. A new sampling method for monitoring the personal exposure to bioaerosol particles has recently been developed and evaluated with bacteria and fungi. In this method, bacterial/fungal aerosol is aspirated and transported through a porous medium, which is submerged into a liquid layer. As the air is split into numerous bubbles, the particles are scavenged by these bubbles and effectively removed. The current feasibility study was initiated to evaluate the efficiency of the new personal sampler prototype ("bubbler") with airborne viable viruses. Two common viral strains, Influenza (stress-sensitive) and Vaccinia (robust), were aerosolized in the test chamber and collected by two identical "bubblers" that operated simultaneously for a duration of upto 5 min. A virus maintenance liquid, proven to be the optimum collecting environment for the test organisms, was used as a collection fluid. After sampling, the collecting fluid was analyzed and the viral recovery rate was determined. The overall recovery (affected not only by the sampling but also by the aerosolization and the aerosol transport) was 20% for Influenza virus and 89% for Vaccinia virus. The new sampling method was found feasible for the collection and enumeration of robust airborne viruses. © 2004 Elsevier Ltd. All rights reserved.

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  • 1. Version of Record от 2021-04-27

Метаданные

Об авторах
  • I. Agranovski
    School of Environmental Engineering, Faculty of Environmental Sciences, Griffith University, Brisbane, QLD 4111, Australia
  • A. Safatov
    State Res. Ctr. Virol. Biotech. Vec., Koltsovo, Novosibirsk Region 630559, Russian Federation
  • A. Borodulin
    Ctr. for Medical and Health Physics, Queensland University of Technology, Brisbane, QLD, Australia
  • O. Pyankov
    Ctr. Hlth.-Related Aerosol Studs., University of Cincinnati, Cincinnati, OH 45267-0056, United States
  • V. Petrishchenko
  • A. Sergeev
  • A. Sergeev
  • V. Agranovski
  • S. Grinshpun
Название журнала
  • Journal of Aerosol Science
Том
  • 36
Выпуск
  • 5-6
Страницы
  • 609-617
Ключевые слова
  • Aerosols; Bacteria; Diseases; Fungi; Porosity; Scavenging; Airborne viruses; Bioaerosols; Influenza; Vaccinia; Viruses; sampler; virus; aerosol; air sampler; air sampling; Arbovirus; article; cell viability; chick embryo; controlled study; embryo; environment; evaluation; Influenza virus; liquid; nonhuman; priority journal; sensitivity analysis; stress; Vaccinia virus; virus strain; airborne virus; Bacteria (microorganisms); Fungi; Influenza virus; Vaccinia; Vaccinia virus
Издатель
  • Elsevier Ltd
Тип документа
  • journal article
Источник
  • scopus