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Статья
Development and verification of real-time PCR assay for identification of viral agents causing acute respiratory infections in human beings
2021
A multiplex polymerase chain reaction (PCR) for identification of four viruses causing acute respiratory diseases in human beings was developed. The analytical sensitivity of developed RT-PCR for identification of adenovirus, respiratory-syncytial virus, flu viruses types A and B, and actual subtypes of type A flu virus (seasonal and pandemic variants H1N1, seasonal H3N2, and viruses of bird flu that are pathogenic to human beings H5 and H7) was 1 × 103 genome equivalents per milliliter. Diagnostic sensitivity for flu virus type A and B, and also subtypes H1 (seasonal H1N1, pandemic variant of H1N1 of year 2009), H3, H5 was 1 × 103-104 viral particles per milliliter. The method developed has high specificity and does not have positive signal in experiments with DNA/cDNA of human beings and viral DNA. We have studied 50 samples using the developed set. Etiology was defined in 33 samples. © 2013 Allerton Press, Inc.
Цитирование
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Версии
- 1. Version of Record от 2021-04-27
Метаданные
Название журнала
- Molecular Genetics, Microbiology and Virology
Том
- 28
Выпуск
- 4
Страницы
- 168-174
Ключевые слова
- complementary DNA; virus DNA; virus RNA; Adenovirus; article; clinical article; controlled study; human; Influenza virus A; Influenza virus A H1N1; Influenza virus A H3N2; Influenza virus A H5N1; Influenza virus A H7N8; Influenza virus B; nonhuman; nucleotide sequence; priority journal; real time polymerase chain reaction; Respiratory syncytial pneumovirus; respiratory tract infection; sensitivity and specificity; sequence analysis; virus detection; virus genome; virus identification; virus infection; virus particle
Тип документа
- journal article
Тип лицензии Creative Commons
- CC
Правовой статус документа
- Свободная лицензия
Источник
- scopus
