Статья

Novel approach to activity evaluation for release-active forms of anti-interferon-gamma antibodies based on enzyme-linked immunoassay

E. Gavrilova, S. Bobrovnik, G. Sherriff, A. Myslivets, S. Tarasov, O. Epstein,
2021

Selection of a suitable assay to measure the activity of drug agents based on release-active forms of anti-interferon-gamma antibodies (RA forms of Abs) is an important step forward in the investigation of such agents. In this study, the enzymelinked immunosorbent assay was utilized to examine the effect of RA forms of Abs specific for human interferon gamma on the interaction between monoclonal anti-interferon gamma antibodies and recombinant human interferon gamma. The experimental data and the results obtained by using relevant mathematical analysis showed that such RA forms of Abs are able to modulate the monoclonal antibody interaction with both soluble and immobilized (to the assay plate well) interferon gamma. These data demonstrated the importance of using relatively low concentrations of both soluble and plate-immobilized interferon gamma to detect the effects of RA forms of Abs to interferon gamma on the binding of monoclonal antibodies to interferon gamma. It has been suggested that the observed influence of RA forms of Abs on 'antibody-antigen' interaction could be used to detect and analyze the activity of drugs containing RA forms of Abs. © 2014 Gavrilova et al.

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Версии

  • 1. Version of Record от 2021-04-27

Метаданные

Об авторах
  • E. Gavrilova
    OOO NPF MATERIA MEDICA HOLDING, Moscow, Russian Federation
  • S. Bobrovnik
    Department of Molecular Immunology, Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine
  • G. Sherriff
    Ab Biotechnology Limited, Penicuik, United Kingdom
  • A. Myslivets
  • S. Tarasov
  • O. Epstein
Название журнала
  • PLoS ONE
Том
  • 9
Выпуск
  • 5
Страницы
  • -
Ключевые слова
  • gamma interferon; gamma interferon antibody; gamma interferon; IFNG protein, human; immobilized protein; monoclonal antibody; antibody affinity; antibody detection; antibody specificity; antigen binding; article; concentration (parameters); controlled study; enzyme linked immunosorbent assay; molecular interaction; chemistry; enzyme linked immunosorbent assay; human; immunology; procedures; solubility; Antibodies, Monoclonal; Enzyme-Linked Immunosorbent Assay; Humans; Immobilized Proteins; Interferon-gamma; Solubility
Издатель
  • Public Library of Science
Тип документа
  • Retracted
Тип лицензии Creative Commons
  • CC
Правовой статус документа
  • Свободная лицензия
Источник
  • scopus