Статья

Allelic variants of NRF2 and TLR9 genes in critical illness

A. Chumachenko, A. Myazin, A. Kuzovlev, A. Gaponov, A. Tutelyan, L. Porokhovnik, A. Golubev, V. Pisarev,
2021

Aim of the study. To elucidate the association of allelic variants of single nucleotide polymorphism in NRF2 (rs6726395, 177238501A>G) and TLR9 (rs352162, 52218953T>C) genes, each gene separately and in their com bination, with peculiarities of the course of critical conditions during lung infection. Materials and methods. DNA from 86 post-operative patients and oncologic patients was genotyped in an allele specific fashion using tetra-primer polymerase chain reaction followed by gel electrophoresis analysis of products. Results. It has been found that septic shock patients with NRF2 177238501A>G GG genotype had increased mortality and higher APACHE II score and developed non-responsive edema more frequently. Patients with NRF2 177238501A>G GG/TLR9 52218953T>C CC genotype combination developed septic shock and nosocomial pneumonia more rarely. Conclusion. The homozygous NRF2 177238501A>G (GG) allele combination is unfavorable for the course and outcome of critical conditions only in combination with TLR9 52218953T>C СT or TLR9 52218953T>C TT alleles in septic shock patients. At the same time, the combination of TLR9 52218953T>C СС alleles in the same patients with ‘unfavorable’ NRF2 177238501A>G GG protects against development of septic shock and nosoco mial pneumonia. © 2016, V.A. Negovsky Research Institute of General Reanimatology. All Rights reserved.

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  • 1. Version of Record от 2021-04-27

Метаданные

Об авторах
  • A. Chumachenko
    V. A. Negovsky Research Institute of General Reanimatology, 25, Petrovka Str., Build. 2, Moscow, 107031, Russian Federation
  • A. Myazin
    D. Rogachev Federal scientific clinical centre of pediatric Hematology, Oncology and Immunology, Ministry of Healthcare of Russia, 1, Samora Mashela Str., GSP-7, Moscow, 117997, Russian Federation
  • A. Kuzovlev
    Central Research Institute of Epidemiology, Rospotrebnadzor, 3a, Novogireevskaya Str., Moscow, 111123, Russian Federation
  • A. Gaponov
    I.M. Sechenov First Moscow State Medical University, Ministry of Healthcare of Russia, 8, Trubetskaya St., Build. 2, Moscow, 119991, Russian Federation
  • A. Tutelyan
    Medical Genetics Research Center, 1, Moskvorechye Str., Moscow, 115478, Russian Federation
  • L. Porokhovnik
  • A. Golubev
  • V. Pisarev
Название журнала
  • Obshchaya Reanimatologiya
Том
  • 12
Выпуск
  • 4
Страницы
  • 8-23
Издатель
  • V.A. Negovsky Research Institute of General Reanimatology
Тип документа
  • journal article
Тип лицензии Creative Commons
  • CC
Правовой статус документа
  • Свободная лицензия
Источник
  • scopus