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Статья
The use of loop-mediated isothermal DNA amplification for the detection and identification of the anthrax pathogen
2021
The results of detection and identification of Bacillus anthracis strains in loop-mediated isothermal DNA amplification (LAMP) reaction performed under optimized conditions with original primers and thermostable DNA polymerase are presented. Reproducible LAMP-based detection of chromosomal and plasmid DNA targets specific for B. anthracis strains has been demonstrated. No cross reactions with DNA from bacterial strains of other species of the B. cereus group were detected. The development of tests for anthrax-pathogen detection based on the optimized reaction of loop isothermal DNA amplification is planned. These tests will be convenient for clinical studies and field diagnostics due to the absence of requirements for sophisticated equipment. © 2017, Allerton Press, Inc.
Цитирование
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Версии
- 1. Version of Record от 2021-04-27
Метаданные
Название журнала
- Molecular Genetics, Microbiology and Virology
Том
- 32
Выпуск
- 2
Страницы
- 100-108
Ключевые слова
- bacterial DNA; DNA fragment; DNA polymerase; plasmid DNA; anthrax; Article; Bacillus anthracis; Bacillus cereus; Bacillus thuringiensis; bacterial strain; bacterium detection; bacterium identification; controlled study; DNA isolation; loop mediated isothermal amplification; nonhuman; polymerase chain reaction; priority journal
Издатель
- Allerton Press Incorporation
Тип документа
- journal article
Тип лицензии Creative Commons
- CC
Правовой статус документа
- Свободная лицензия
Источник
- scopus
