Поиск по репозиторию
Статья
Genome analysis of E. coli isolated from Crohn's disease patients
2021
Background: Escherichia coli (E. coli) has been increasingly implicated in the pathogenesis of Crohn's disease (CD). The phylogeny of E. coli isolated from Crohn's disease patients (CDEC) was controversial, and while genotyping results suggested heterogeneity, the sequenced strains of E. coli from CD patients were closely related. Results: We performed the shotgun genome sequencing of 28 E. coli isolates from ten CD patients and compared genomes from these isolates with already published genomes of CD strains and other pathogenic and non-pathogenic strains. CDEC was shown to belong to A, B1, B2 and D phylogenetic groups. The plasmid and several operons from the reference CD-associated E. coli strain LF82 were demonstrated to be more often present in CDEC genomes belonging to different phylogenetic groups than in genomes of commensal strains. The operons include carbon-source induced invasion GimA island, prophage I, iron uptake operons I and II, capsular assembly pathogenetic island IV and propanediol and galactitol utilization operons. Conclusions: Our findings suggest that CDEC are phylogenetically diverse. However, some strains isolated from independent sources possess highly similar chromosome or plasmids. Though no CD-specific genes or functional domains were present in all CD-associated strains, some genes and operons are more often found in the genomes of CDEC than in commensal E. coli. They are principally linked to gut colonization and utilization of propanediol and other sugar alcohols. © 2017 The Author(s).
Цитирование
Похожие публикации
Версии
- 1. Version of Record от 2021-04-27
Метаданные
Название журнала
- BMC Genomics
Том
- 18
Выпуск
- 1
Страницы
- -
Ключевые слова
- bacteriocin; antiinfective agent; adult; Article; bacterial genome; bacterial strain; bacteriophage; bacterium isolation; cecum; clinical article; controlled study; Crohn disease; enteropathogenic Escherichia coli; Escherichia coli; feces analysis; female; genetic variability; genome analysis; genotype; human; human tissue; ileum; intestine biopsy; male; middle aged; operon; phylogeny; plasmid; sequence analysis; shotgun genome sequencing; sigmoid; young adult; antibiotic resistance; biosynthesis; Crohn disease; drug effects; Escherichia coli; genetic variation; genetics; genomics; metabolism; microbiology; physiology; Adult; Anti-Bacterial Agents; Bacteriocins; Crohn Disease; Drug Resistance, Bacterial; Escherichia coli; Female; Genetic Variation; Genomics; Humans; Male; Middle Aged; Phylogeny; Young Adult
Издатель
- BioMed Central Ltd.
Тип документа
- journal article
Тип лицензии Creative Commons
- CC
Правовой статус документа
- Свободная лицензия
Источник
- scopus
