Статья

The Development and Study of Recombinant Immunoglobulin A to Hemagglutinins of the Influenza Virus

T. Aliev, I. Dement’yeva, V. Toporova, V. Argentova, L. Pozdnyakova, M. Bokov, Y. Votchitseva, D. Dolgikh, S. Varfolomeyev, P. Sveshnikov, M. Kirpichnikov,
2021

We obtained recombinant variants of human antibody FI6 broadly specific to hemagglutinins of the influenza A virus. On the basis of a bi-promoter (CMV, hEF1-HTLV) vector, we developed genetic constructs for the expression of the heavy and light chains of the immunoglobulins of IgA1-, IgA2m1-, and IgG-isotypes. Following transfection and selection, stable Chinese hamster ovary (CHO) cell lines were produced. The antibodies of IgA1-, IgA2m1-, and IgG-isotypes were purified from culture media. We performed an immunochemical characterization and studied their interactions with influenza A strains of the H1N1- and H3N2-subtypes. It was shown that recombinant FI6 variants of the IgA-isotype retain the properties of the parental IgG antibody to demonstrate specificity to all the strains tested. The strongest binding was observed for the H1N1 subtype, which belongs to hemagglutinins of phylogenetic group I. © 2018 Park-media, Ltd. This is an open access article distributed under the Creative Commons Attribution License,which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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  • 1. Version of Record от 2021-04-27

Метаданные

Об авторах
  • T. Aliev
    Lomonosov Moscow State University, Department of Chemistry, Leninskie gory 1, bldg. 3, Moscow, 119991, Russian Federation
  • I. Dement’yeva
    Russian Research Center for Molecular Diagnostics and Therapy, Simferopolsky Blvd. 8, Moscow, 117149, Russian Federation
  • V. Toporova
    Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str. 16/10, Moscow, 117997, Russian Federation
  • V. Argentova
    Lomonosov Moscow State University, Faculty of Biology, Leninskie gory 1, bldg. 12, Moscow, 119991, Russian Federation
  • L. Pozdnyakova
  • M. Bokov
  • Y. Votchitseva
  • D. Dolgikh
  • S. Varfolomeyev
  • P. Sveshnikov
  • M. Kirpichnikov
Название журнала
  • Acta Naturae
Том
  • 10
Выпуск
  • 2
Страницы
  • 30-36
Издатель
  • Acta Naturae
Тип документа
  • journal article
Тип лицензии Creative Commons
  • CC
Правовой статус документа
  • Свободная лицензия
Источник
  • scopus