COVID-19.рф: ИНФОРМАЦИЯ ПРОТИВ ПАНДЕМИИ

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Comparative immunological study in mice of inactivated influenza vaccines used in the Russian immunization program

A. Shanko, M. Shuklina, A. Kovaleva, Y. Zabrodskaya, I. Vidyaeva, A. Shaldzhyan, A. Fadeev, A. Korotkov, M. Zaitceva, L. Stepanova, L. Tsybalova, L. Kordyukova, A. Katlinski,
2021
https://doi.org/10.3390/vaccines8040756

A series of commercial inactivated influenza vaccines (IIVs) used in the Russian National Immunization Program were characterized to evaluate their protective properties on an animal model. Standard methods for quantifying immune response, such as hemagglutination inhibition (HAI) assay and virus neutralization (VN) assay, allowed us to distinguish the immunogenic effect of various IIVs from that of placebo. However, these standard approaches are not suitable to determine the role of various vaccine components in immune response maturation. The expanded methodological base including an enzyme-linked immunosorbent assay (ELISA) and a neuraminidase ELISA (NA-ELISA) helped us to get wider characteristics and identify the effectiveness of various commercial vaccines depending on the antigen content. Investigations conducted showed that among the IIVs tested, Ultrix®, Ultrix® Quadri and VAXIGRIP® elicit the most balanced immune response, including a good NA response. For Ultrix®, Ultrix® Quadri, and SOVIGRIPP® (FORT LLC), the whole-virus specific antibody subclass IgG1, measured in ELISA, seriously prevailed over IgG2a, while, for VAXIGRIP® and SOVIGRIPP® (NPO Microgen JSC) preparations, the calculated IgG1/IgG2a ratio was close to 1. So, the immune response varied drastically across different commercial IIVs injected in mice. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

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Источник

Версии

  • 1. Version of Record от 2021-04-27

Метаданные

Об авторах
  • A. Shanko
    Research and Development Department, FORT LLC, Moscow, 119435, Russian Federation
  • M. Shuklina
    N. F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ivanovsky Institute of Virology, Moscow, 123098, Russian Federation
  • A. Kovaleva
    WHO National Influenza Center, Smorodintsev Research Institute of Influenza, Saint-Petersburg, 197376, Russian Federation
  • Y. Zabrodskaya
    Peter the Great Saint-Petersburg Polytechnical University, Saint-Petersburg, 194064, Russian Federation
  • I. Vidyaeva
    Petersburg Nuclear Physics Institute named by B. P. Konstantinov of National Research Center “Kurchatov Institute”, Gatchina, 188300, Russian Federation
  • A. Shaldzhyan
    Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119991, Russian Federation
  • A. Fadeev
    FORT LLC, Moscow, 119435, Russian Federation
  • A. Korotkov
  • M. Zaitceva
  • L. Stepanova
  • L. Tsybalova
  • L. Kordyukova
  • A. Katlinski
Название журнала
  • Vaccines
Том
  • 8
Выпуск
  • 4
Страницы
  • 1-17
Ключевые слова
  • agarose; immunoglobulin G1; immunoglobulin G2a; inactivated influenza vaccine; influenza vaccine; neutralizing antibody; placebo; polysorbate 20; quadri; sovigripp; tetramethylbenzidine; trypsin; ultrix; unclassified drug; virus vaccine; animal cell; animal experiment; animal model; antibody response; Article; Bagg albino mouse; enzyme activity; enzyme linked immunosorbent assay; enzyme linked immunospot assay; euthanasia; female; hemagglutination; hemagglutination inhibition; immune response; immunization; immunodiffusion; influenza A (H1N1); mass spectrometry; matrix assisted laser desorption ionization time of flight mass spectrometry; MDCK cell line; mouse; nonhuman; polyacrylamide gel electrophoresis; single radial immunodiffusion assay; slot blot hybridization; virus neutralization; virus replication
Издатель
  • MDPI AG
Тип документа
  • journal article
Тип лицензии Creative Commons
  • CC
Правовой статус документа
  • Свободная лицензия
Источник
  • scopus
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