Поиск по репозиторию
Статья
Construction and immunogenicity of a novel multivalent vaccine prototype based on conserved influenza virus antigens
2021
Influenza, an acute, highly contagious respiratory disease, remains a significant threat to public health. More effective vaccination strategies aimed at inducing broad cross-protection not only against seasonal influenza variants, but also zoonotic and emerging pandemic influenza strains are urgently needed. A number of conserved protein targets to elicit such cross-protective immunity have been under investigation, with long alpha-helix (LAH) from hemagglutinin stalk and ectodomain of matrix protein 2 ion channel (M2e) being the most studied ones. Recently, we have reported the three-dimensional structure and some practical applications of LAH expressed in Escherichia coli system (referred to as tri-stalk protein). In the present study, we investigated the immunogenicity and efficacy of a panel of broadly protective influenza vaccine prototypes based on both influenza tri-stalk and triple M2e (3M2e) antigens integrated into phage AP205 virus-like particles (VLPs). While VLPs containing the 3M2e alone induced protection against standard homologous and heterologous virus challenge in mice, only the combination of both conserved influenza antigens into a single VLP fully protected mice from a high-dose homologous H1N1 influenza infection. We propose that a combination of genetic fusion and chemical coupling techniques to expose two different foreign influenza antigens on a single particle is a perspective approach for generation of a broadly-effective vaccine candidate that could protect against the constantly emerging influenza virus strains. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Цитирование
Похожие публикации
Версии
- 1. Version of Record от 2021-04-27
Метаданные
Название журнала
- Vaccines
Том
- 8
Выпуск
- 2
Страницы
- -
Ключевые слова
- epitope; gamma interferon; hemagglutinin; immunoglobulin A; immunoglobulin G; immunoglobulin G1; influenza vaccine; mu opiate receptor; Pneumococcus vaccine; recombinant vaccine; tumor necrosis factor; animal experiment; animal model; antibody dependent cellular cytotoxicity; antibody response; antigen binding; Article; CD4+ T lymphocyte; CD8+ T lymphocyte; controlled study; cytotoxic T lymphocyte; cytotoxicity assay; degranulation assay; enzyme immunoassay; enzyme linked immunosorbent assay; enzyme linked immunospot assay; expression vector; female; flow cytometry; genetic reassortment; immune response; influenza; Influenza virus; LD50; mouse; nonhuman; protein analysis; protein purification; vaccination; vaccine immunogenicity; virus neutralization; virus particle
Издатель
- MDPI AG
Тип документа
- journal article
Тип лицензии Creative Commons
- CC
Правовой статус документа
- Свободная лицензия
Источник
- scopus
