Статья

Epidemiological and molecular characterization of Streptococcus pneumoniae carriage strains in pre-school children in Arkhangelsk, northern European Russia, prior to the introduction of conjugate pneumococcal vaccines

S. Vorobieva, A. Furberg, H. Slotved, T. Bazhukova, B. Haldorsen, D. Caugant, A. Sundsfjord, P. Valentiner-Branth, G. Simonsen,
2021

Background: The 13-valent Pneumococcal Conjugate Vaccine (PCV-13) was introduced in the National Immunization Programme (NIP) schedule in Russia in March 2014. Previously, the 7-valent Pneumococcal Conjugate Vaccine (PCV-7) was marketed in Russia in 2009 but has never been offered for mass vaccination. A carriage study was performed among children in Arkhangelsk in 2006. The objective was to determine the prevalence of carriage, serotype distribution, antimicrobial susceptibility and the molecular structure of Streptococcus pneumoniae strains before marketing and introduction of PCV-13. Methods: A cross-sectional study was conducted on a cluster-randomized sample of children and a self-administrated questionnaire for parents/guardians. Nasopharyngeal samples were collected from 438 children younger than 7 years attending nurseries and kindergartens in the Arkhangelsk region, Russia. Detailed demographic data, as well as information about the child's health, traveling, exposure to antimicrobials within the last 3 months and anthropometric measurements were collected for all study subjects. Variables extracted from the questionnaire were analysed using statistic regression models to estimate the risk of carriage. All pneumococcal isolates were examined with susceptibility testing, serotyping and multilocus sequence typing. Results: The overall prevalence of asymptomatic carriage was high and peaking at 36 months with a rate of 57%. PCV-13 covered 67.3% of the detected strains. High rates of non-susceptibility to penicillin, macrolides and multidrug resistance were associated with specific vaccine serotypes, pandemic clones, and local sequence types. Nine percent of isolates represented three globally disseminated disease-associated pandemic clones; penicillin- A nd macrolide-resistant clones NorwayNT-42 and Poland6B-20, as well as penicillin- A nd macrolide-susceptible clone Netherlands3-31. A high level of antimicrobial consumption was noted by the study. According to the parent's reports, 89.5% of the children used at least one antimicrobial regime since birth. None of the hypothesised predictors of S. pneumoniae carriage were statistically significant in univariable and multivariable logistic models. Conclusions: The study identified a high coverage of the PCV-13-vaccine, but serotype replacement and expansion of globally disseminated disease-associated clones with non-vaccine serotypes may be expected. Further surveillance of antimicrobial resistance and serotype distribution is therefore required. © 2020 The Author(s).

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  • 1. Version of Record от 2021-04-27

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Об авторах
  • S. Vorobieva
    Research Group for Host-Microbe Interaction, Department of Medical Biology, Faculty of Health Sciences, UiT-The Arctic University of Norway, Tromsø, Norway
  • A. Furberg
    Department of Virus and Microbiological Special Diagnostics, Division of Infectious Disease Preparedness, Statens Serum Institut, Artillerivej 5, Copenhagen S, DK-2300, Denmark
  • H. Slotved
    Faculty of Health and Social Sciences, Molde University College, Molde, Norway
  • T. Bazhukova
    Department of Microbiology and Infection Control, University Hospital of North Norway, Tromsø, Norway
  • B. Haldorsen
    Department of Bacteria, Parasites and Fungi, Division of Infectious Disease Preparedness, Statens Serum Institute, Copenhagen, Denmark
  • D. Caugant
    Department of Clinical Biochemistry, Microbiology and Laboratory Diagnostics, Northern State Medical University, Arkhangelsk, Russian Federation
  • A. Sundsfjord
    Norwegian National Advisory Unit on Detection of Antimicrobial Resistance, Department of Microbiology and Infection Control, University Hospital of North Norway, Tromsø, Norway
  • P. Valentiner-Branth
    Division of Infection Control and Environmental Health, Norwegian Institute of Public Health, Oslo, Norway
  • G. Simonsen
    Infectious Disease Epidemiology and Prevention, Statens Serum Institut, Copenhagen, Denmark
Название журнала
  • BMC Infectious Diseases
Том
  • 20
Выпуск
  • 1
Страницы
  • -
Ключевые слова
  • cefotaxime; cefuroxime; ciprofloxacin; clindamycin; cotrimoxazole; erythromycin; levofloxacin; macrolide; meropenem; moxifloxacin; oxacillin; penicillin derivative; Pneumococcus vaccine; tetracycline; 13-valent pneumococcal vaccine; antiinfective agent; macrolide; penicillin derivative; Pneumococcus vaccine; vaccine; antibiotic resistance; Article; bacterial strain; bacterium carrier; child; childhood; clone; cross-sectional study; drug use; female; gene sequence; human; major clinical study; male; molecular biology; multidrug resistance; multilocus sequence typing; Netherlands; nonhuman; Norway; pandemic; pneumococcal infection; Poland; preschool child; prevalence; Russian Federation; serotype; statistical significance; Streptococcus pneumoniae; drug effect; genetics; heterozygote; immunology; infant; isolation and purification; microbial sensitivity test; microbiology; nasopharynx; pneumococcal infection; serotyping; Streptococcus pneumoniae; Anti-Bacterial Agents; Carrier State; Child; Child, Preschool; Cross-Sectional Studies; Drug Resistance, Multiple, Bacterial; Female; Humans; Infant; Macrolides; Male; Microbial Sensitivity Tests; Multilocus Sequence Typing; Nasopharynx; Penicillins; Pneumococcal Infections; Pneumococcal Vaccines; Prevalence; Russia; Serogroup; Serotyping; Streptococcus pneumoniae; Vaccines, Conjugate
Издатель
  • BioMed Central Ltd.
Тип документа
  • journal article
Тип лицензии Creative Commons
  • CC
Правовой статус документа
  • Свободная лицензия
Источник
  • scopus