RT - journal article
SR - Electronic
T1 - Longitudinal high-throughput TCR repertoire profiling reveals the dynamics of T-cell  memory formation after mild COVID-19 infection.
JF - eLife
SP - 2021-07-23
DO - 10.7554/eLife.63502
A1 - Minervina, Anastasia A
A1 - Komech, Ekaterina A
A1 - Titov, Aleksei
A1 - Bensouda, Koraichi Meriem
A1 - Rosati, Elisa
A1 - Mamedov, Ilgar Z
A1 - Franke, Andre
A1 - Efimov, Grigory A
A1 - Chudakov, Dmitriy M
A1 - Mora, Thierry
A1 - Walczak, Aleksandra M
A1 - Lebedev, Yuri B
A1 - Pogorelyy, Mikhail V
YR - 2021
UL - https://covid19.neicon.ru/publication/10163
AB - COVID-19 is a global pandemic caused by the SARS-CoV-2 coronavirus. T cells play a

key role in the adaptive antiviral immune response by killing infected cells and

facilitating the selection of virus-specific antibodies. However, neither the

dynamics and cross-reactivity of the SARS-CoV-2-specific T-cell response nor the

diversity of resulting immune memory is well understood. In this study, we use

longitudinal high-throughput T-cell receptor (TCR) sequencing to track changes in

the T-cell repertoire following two mild cases of COVID-19. In both donors, we

identified CD4(+) and CD8(+) T-cell clones with transient clonal expansion after

infection. We describe characteristic motifs in TCR sequences of COVID-19-reactive

clones and show preferential occurrence of these motifs in publicly available large

dataset of repertoires from COVID-19 patients. We show that in both donors, the

majority of infection-reactive clonotypes acquire memory phenotypes. Certain T-cell

clones were detected in the memory fraction at the pre-infection time point,

suggesting participation of pre-existing cross-reactive memory T cells in the immune

response to SARS-CoV-2.