%0 journal article
%A Minervina, Anastasia A
%A Komech, Ekaterina A
%A Titov, Aleksei
%A Bensouda, Koraichi Meriem
%A Rosati, Elisa
%A Mamedov, Ilgar Z
%A Franke, Andre
%A Efimov, Grigory A
%A Chudakov, Dmitriy M
%A Mora, Thierry
%A Walczak, Aleksandra M
%A Lebedev, Yuri B
%A Pogorelyy, Mikhail V
%T Longitudinal high-throughput TCR repertoire profiling reveals the dynamics of T-cell  memory formation after mild COVID-19 infection.
%D 2021
%R 10.7554/eLife.63502
%J eLife
%X COVID-19 is a global pandemic caused by the SARS-CoV-2 coronavirus. T cells play a

key role in the adaptive antiviral immune response by killing infected cells and

facilitating the selection of virus-specific antibodies. However, neither the

dynamics and cross-reactivity of the SARS-CoV-2-specific T-cell response nor the

diversity of resulting immune memory is well understood. In this study, we use

longitudinal high-throughput T-cell receptor (TCR) sequencing to track changes in

the T-cell repertoire following two mild cases of COVID-19. In both donors, we

identified CD4(+) and CD8(+) T-cell clones with transient clonal expansion after

infection. We describe characteristic motifs in TCR sequences of COVID-19-reactive

clones and show preferential occurrence of these motifs in publicly available large

dataset of repertoires from COVID-19 patients. We show that in both donors, the

majority of infection-reactive clonotypes acquire memory phenotypes. Certain T-cell

clones were detected in the memory fraction at the pre-infection time point,

suggesting participation of pre-existing cross-reactive memory T cells in the immune

response to SARS-CoV-2.
%U https://covid19.neicon.ru/publication/10163