@article{Minervina2021-07-23,
author = {A. A. Minervina, E. A. Komech, A. Titov, K. M. Bensouda, E. Rosati, I. Z. Mamedov, A. Franke, G. A. Efimov, D. M. Chudakov, T. Mora, A. M. Walczak, Y. B. Lebedev, M. V. Pogorelyy},
title = {Longitudinal high-throughput TCR repertoire profiling reveals the dynamics of T-cell  memory formation after mild COVID-19 infection.},
year = {2021},
doi = {10.7554/eLife.63502},
publisher = {NP «NEICON»},
abstract = {COVID-19 is a global pandemic caused by the SARS-CoV-2 coronavirus. T cells play a

key role in the adaptive antiviral immune response by killing infected cells and

facilitating the selection of virus-specific antibodies. However, neither the

dynamics and cross-reactivity of the SARS-CoV-2-specific T-cell response nor the

diversity of resulting immune memory is well understood. In this study, we use

longitudinal high-throughput T-cell receptor (TCR) sequencing to track changes in

the T-cell repertoire following two mild cases of COVID-19. In both donors, we

identified CD4(+) and CD8(+) T-cell clones with transient clonal expansion after

infection. We describe characteristic motifs in TCR sequences of COVID-19-reactive

clones and show preferential occurrence of these motifs in publicly available large

dataset of repertoires from COVID-19 patients. We show that in both donors, the

majority of infection-reactive clonotypes acquire memory phenotypes. Certain T-cell

clones were detected in the memory fraction at the pre-infection time point,

suggesting participation of pre-existing cross-reactive memory T cells in the immune

response to SARS-CoV-2.},
URL = {https://covid19.neicon.ru/publication/10163},
eprint = {https://covid19.neicon.ru/files/3825},
journal = {eLife},
}