Anthropogenic effects on the ecological situation can change the genome of closely related animal and human viruses. This situation threatens the emergence of uncontrolled epidemic and epizootic processes. The use of chemically synthesized antiviral drugs causes the appearance of resistant mutant types of herpes viruses and adenoviruses. Then chemical drugs cause systemic complications in the therapy of adenovirosis and herpes diseases of humans and animals. The virucidal effect of dialderon (decahydroxyproline-2-decenohydroisoquinoline dimethylaminoethanol albuminate) on the cell infection of MDBK (Madin-Darby bull kidney) cell culture with cattle type 3 adenovirus and herpes virus on the KEC (kidney embryo cow) culture was studied in order to search for harmless antiviral drugs. It was found that dialderone in a dose of 1000 μg/mL did not have cytotoxic effect on MDBK and KEC cells. The dialderone in a dose of 100 μg/mL had no direct virucidal effect on type 3 adenovirus on MDBK cell culture, while inhibit virus replication with infection activity of 2.2 lgTCID50/mL during 48 hours. The drug reduced the number of infected cells to 76.2±0.15% compared to 99.8±0.11% in the control after 96 hours. The test drug inhibit completely the growth of herpes type 1 virus on the culture of KEC cells and reduced in the virus titer by 3.45 lgTCID50/mL. Dialderon reduced the number of infected KEC cells from 98.4±0.15% (in control) to 9.6±0.12% (in the test) during 96 hours. The drug prevented acidification of the medium, lowered the level of malondialdehyde and caused the activation of α-interferon production in the culture of KEC cells.