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Next-Generation Techniques for Discovering Human Monoclonal Antibodies

Molecular Biology volume 51, pages 782–787 (2017)Cite this article

Abstract

Monoclonal antibodies have found wide applications in the treatment of cancer, as well as of autoimmune, infectious, and other diseases. Several dozen new antibodies are currently undergoing different stages of clinical trials, and some of them will soon be added to the list of immunotherapeutic drugs. Most of these antibodies have been generated using hybridoma technology or a phage display. In recent years, new methods of obtaining human monoclonal antibodies have been actively developing. These methods rely on sequencing immunoglobulin genes from B lymphocytes, as well as on the creation of antibody-secreting stable B-cell lines. The term next-generation antibody-discovery platforms has already been established in the literature to refer to these approaches. Our review focuses on describing the results obtained by these methods.

Abbreviations

ELISpot:

enzyme-linked immunospot

NGS:

next-generation sequencing

VH:

variable domain of the heavy chain

VL:

variable domain of the light chain

mAb:

monoclonal antibody

SARS:

severe acute respiratory syndrome.

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Author information

Affiliations

  1. Institute of Immunology, Federal Medical-Biological Agency of Russia, Moscow, 115478, Russia

    A. A. Lushova, M. G. Biazrova & A. V. Filatov

  2. Ivanovsky Institute of Virology, Gamaleya Scientific Research Institute of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, 123098, Russia

    A. G. Prilipov & G. K. Sadykova

  3. Orekhovich Institute of Biomedical Chemistry, Moscow, 119121, Russia

    T. A. Kopylov

Authors
  1. A. A. Lushova
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  2. M. G. Biazrova
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  3. A. G. Prilipov
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  4. G. K. Sadykova
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  5. T. A. Kopylov
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  6. A. V. Filatov
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Corresponding author

Correspondence to A. V. Filatov.

Additional information

Original Russian Text © A.A. Lushova, M.G. Biazrova, A.G. Prilipov, G.K. Sadykova, T.A. Kopylov, A.V. Filatov, 2017, published in Molekulyarnaya Biologiya, 2017, Vol. 51, No. 6, pp. 899–906.

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Lushova, A.A., Biazrova, M.G., Prilipov, A.G. et al. Next-Generation Techniques for Discovering Human Monoclonal Antibodies. Mol Biol 51, 782–787 (2017). https://doi.org/10.1134/S0026893317060103

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Keywords

  • human monoclonal antibodies
  • next-generation sequencing
  • B-cell immortalization
  • immunoglobulins